FFPE Lung Tissue Samples: DNA & RNA Extraction for Lung Cancer Genomics

Both genomic DNA (gDNA) and RNA can be extracted from FFPE lung tissue. The Cambrian FFPE gDNA Isolation Kit yields high-molecular-weight DNA suitable for NGS and PCR, while the Cambrian FFPE RNA Isolation Kit delivers high-integrity RNA (DV200 >70%) for transcriptomics, gene expression profiling, and fusion gene detection in lung cancer research.

Both the FFPE gDNA and RNA Isolation Kits complete the extraction workflow in 28 minutes after lysis. No overnight incubation is required, enabling same-day results. This accelerated workflow is compatible with the Manta automated platform, supporting throughput of up to 32 samples per run without batching pressure.

Yes. The Cambrian FFPE gDNA Isolation Kit delivers high-molecular-weight DNA validated for NGS, PCR-based mutation analysis, and oncology studies. The FFPE RNA Isolation Kit produces RNA with DV200 >70% and A260/280 purity of 1.9–2.1, meeting quality thresholds for RNA-seq, transcriptome studies, and fusion gene detection workflows.

The Cambrian FFPE RNA Isolation Kit requires as few as four 10 µm FFPE tissue curls as input, yielding approximately 5,000 ng of RNA. The gDNA kit is similarly optimized for minimal sample input, making it ideal for oncology workflows where precious archival FFPE tissue blocks must be conserved.

No. The Cambrian FFPE RNA Isolation Kit features integrated DNase treatment that eliminates DNA contamination within the extraction workflow itself. This ensures zero gDNA carry-over, safeguarding the accuracy of downstream RNA-based applications such as gene expression profiling, lncRNA/miRNA research, and immune profiling studies.

Yes. Both kits are designed for use with the Manta automated nucleic acid extraction platform by Cambrian Bioworks. The system supports 1 to 32 samples per run, processes distinct sample types simultaneously, and uses a pre-filled cartridge format. Automation integration support is available directly from the Cambrian Bioworks technical team.