FFPE Tissue Dissociation & DNA/RNA Extraction: Complete Workflow Guide

Both kits complete purification in 28 minutes after lysis — no overnight incubation required. The RNA kit is designed for same-day processing, significantly increasing laboratory throughput. When run on the Manta automated platform, the workflow is further streamlined with minimal hands-on time.

The kit consistently delivers approximately 5,000 ng of RNA (measured by Qubit) from four 10 µm FFPE curls, with DV200 values exceeding 70% and A260/280 purity ratios of 1.9–2.1. RIN values typically range from 1.9 to 3.8, which is expected given the inherently degraded nature of FFPE-derived RNA.

Yes. DNase treatment is fully integrated into the Cambrian FFPE RNA Isolation Kit workflow, ensuring zero DNA contamination in the final eluate. This is critical for accurate downstream applications such as gene expression profiling, RNA-seq, and biomarker studies where genomic DNA carryover would compromise results.

Yes. Both kits are designed for seamless integration with the Manta cartridge-based automated nucleic acid extraction system. The gDNA kit supports 1 to 32 samples per run with adaptable throughput, while the RNA kit is automation-friendly with 96-well format compatibility, making them suitable for high-throughput oncology and diagnostic workflows.

The extracted RNA supports RNA-seq, gene expression profiling, fusion gene detection (e.g., BCR-ABL), lncRNA/miRNA research, tumor subtype classification, and single-cell transcriptomics. Extracted gDNA is validated for NGS, PCR-based mutation analysis, oncology studies, blood cancer diagnostics, infectious disease testing, and reproductive genetics applications.

The FFPE gDNA Isolation Kit is intended for both research and clinical diagnostic laboratory use and is CE-IVD marked under EU Regulation 2017/746 and CDSCO certified for India. The FFPE RNA Isolation Kit is currently designated as RUO (Research Use Only). Regulatory status confirmation is available upon inquiry via the Cambrian Bioworks contact page.