FFPE Tissue Microarrays: DNA/RNA Extraction for Molecular Profiling

Yes, FFPE samples can be used for RNA sequencing and gene expression profiling when the extraction chemistry is optimized for degraded and crosslinked material. The Cambrian FFPE RNA Isolation Kit is designed for FFPE tissue and reports DV200 above 70%, supporting applications such as transcriptome studies, fusion detection, biomarker discovery, and immune profiling.

Input requirements vary by kit, but low-input workflows are available for limited tissue. The Cambrian FFPE RNA Isolation Kit can work with as few as four 10 µm FFPE tissue curls. This makes it suitable for retrospective studies, tumor subtype classification, and other workflows where archived material or small sections must be used efficiently.

Processing time depends on the workflow, but both listed Cambrian FFPE kits support rapid extraction. The RNA kit avoids overnight lysis and enables same-day processing, while the gDNA kit runs in about 28 minutes after lysis. Faster workflows can improve lab throughput and reduce delays in sequencing, PCR, and expression analysis pipelines.

Yes, both products are designed with automation in mind. The RNA kit is compatible with the Manta automated platform and 96-well format systems, while the gDNA kit supports the Manta cartridge-based extraction system with throughput from 1 to 32 samples per run. This helps labs scale processing while maintaining reproducibility across batches.

These FFPE extraction kits support a broad range of downstream molecular applications. The RNA kit is used for gene expression profiling, RNA-seq, fusion gene detection, splice variant analysis, and biomarker studies. The gDNA kit supports PCR, mutation analysis, NGS workflows, oncology studies, reproductive genetics, and infectious disease testing in laboratory settings.

The intended use differs by product. The Cambrian FFPE RNA Isolation Kit is designated RUO, meaning it is for research use only unless further validation is completed. The Cambrian FFPE gDNA Isolation Kit is described for research and clinical diagnostic laboratory use. Labs should confirm regulatory fit and validation requirements for their specific workflow.