Whole Genome DNA Testing Kits: Extraction to Sequencing Workflow Guide

The cost of whole genome sequencing (WGS) varies based on sample type, kit configuration, and sequencing depth. Cambrian Bioworks' extraction kits — including the Whole Blood gDNA, FFPE gDNA, and OncoSeek cfDNA kits — are available on a request-for-quote basis. Pricing is optimized to lower cost per test by up to 56% compared to conventional methods. Contact Cambrian Bioworks for a tailored quote.

Cambrian Bioworks offers dedicated kits for whole blood (200 µL–2 mL), FFPE tissue, and plasma (minimum 1 mL). The Manta system also supports simultaneous processing of multiple distinct sample types in a single run, making it suitable for diverse lab workflows handling clinical and research specimens concurrently.

The Whole Blood gDNA and FFPE gDNA Isolation Kits complete extraction in just 28 minutes on-deck after lysis, with total processing under 30 minutes. The OncoSeek cfDNA Extraction Kit for plasma samples completes the workflow in approximately 55 minutes. Both workflows are designed to minimize hands-on time through pre-filled cartridges and automated bead-based purification.

Yes. All three kits are validated for Next-Generation Sequencing (NGS), PCR-based assays, and microarray analysis. The OncoSeek cfDNA kit is additionally optimized for digital PCR (ddPCR), liquid biopsy workflows, and minimal residual disease (MRD) detection. DNA outputs meet purity specifications (A260/280: 1.8–2.0) required for sensitive downstream applications.

The Whole Blood gDNA Isolation Kit delivers yields of 6–15 ng/µL with A260/280 ratios of 1.8–2.0 and A260/230 ratios of 2.0–2.2 from a 200 µL blood input. The OncoSeek cfDNA kit provides up to 5–20× higher cfDNA yield compared to leading competitors using just 1 mL of plasma, with minimal genomic DNA contamination for clean, sequencing-ready output.

Yes. The Cambrian FFPE gDNA Isolation Kit is specifically designed for tumor tissue samples, supporting mutation analysis, tumor content assessment, and oncology NGS workflows. The OncoSeek cfDNA Kit enables liquid biopsy applications including ctDNA detection, MRD monitoring, and early cancer detection research. Both are trusted by institutions like Tata Memorial Hospital and ACTREC.