gDNA Removal Columns for RT-qPCR & RNA Sequencing: Why Extraction Matters

Residual genomic DNA in RNA preparations can produce false-positive signals in RT-qPCR by amplifying alongside cDNA, and introduces noise in RNA-Seq data by generating non-transcriptomic reads. Integrated DNase treatment, as included in Cambrian Bioworks kits, ensures zero DNA contamination, protecting expression profiling accuracy and reducing the need for additional cleanup steps.

The Cambrian Blood RNA Isolation Kit delivers RNA Integrity Numbers (RIN) of 8.6–9.7, validated on the Agilent TapeStation system, making it suitable for sensitive transcriptomics. The FFPE RNA Isolation Kit achieves DV200 values consistently above 70%, which is the standard metric for fragmented FFPE-derived RNA used in RNA-Seq and expression profiling.

Yes. Both kits are designed for automation-friendly workflows. The Cambrian Blood RNA Isolation Kit runs on the Manta automated platform with a 50-minute on-deck time using pre-filled, non-reusable cartridges. The FFPE RNA Isolation Kit is also adaptable to the Manta platform and 96-well format systems, enabling high-throughput RNA extraction for oncology and diagnostics labs.

The Cambrian Blood RNA Isolation Kit processes whole blood and cell cultures with input volumes of 0.5 mL to 2 mL. The FFPE RNA Isolation Kit is optimized for formalin-fixed paraffin-embedded tissue, requiring as few as four 10 µm tissue curls. Together, these kits cover key clinical and research sample types used in oncology, biomarker discovery, and transcriptomics.

The Blood RNA Isolation Kit yields an average of 2.17 µg from 0.5 mL, 5.64 µg from 1 mL, and 9.61 µg from 2 mL of whole blood, with yield scaling linearly with input volume. The FFPE RNA Isolation Kit delivers approximately 5,000 ng (Qubit) from four 10 µm FFPE tissue curls, even from small or over-fixed archival specimens.

The Cambrian Blood RNA Isolation Kit is intended for both research and clinical diagnostic use. The FFPE RNA Isolation Kit is currently designated Research Use Only (RUO). Cambrian Bioworks holds ISO 13485 certification and a CE-IVD mark for clinical IVD applications, and regulatory status for specific products can be confirmed by contacting their team directly.