cfDNA Isolation Buffers & Magnetic Bead Kits for Plasma Extraction

The purified cfDNA from the OncoSeek kit is compatible with a broad range of downstream applications, including next-generation sequencing (NGS), quantitative PCR (qPCR), and digital PCR (ddPCR). It is specifically optimized for liquid biopsy assay development, MRD detection, mutation profiling, and ctDNA analysis in oncology research workflows.

Bead-based purification uses magnetic beads to selectively bind cfDNA under optimized buffer conditions, enabling automation, consistent binding efficiency, and minimal genomic DNA contamination. Unlike column-based methods, bead-based workflows can be integrated into automated liquid handlers, reducing variability and processing time to approximately 55 minutes per run.

The OncoSeek cfDNA Extraction Kit is optimized to preferentially capture 150–200 bp cfDNA fragments, which correspond to tumor-derived circulating tumor DNA (ctDNA). This size-selective enrichment improves detection sensitivity for low-abundance ctDNA, which is critical for minimal residual disease (MRD) detection and early cancer detection research workflows.

The OncoSeek cfDNA Extraction Kit is currently designated For Research Use Only (RUO) and is intended for oncology research and liquid biopsy applications. Cambrian Bioworks does hold ISO 13485 certification and a CE-IVD mark for its product portfolio. For specific regulatory status applicable to your region, please contact Cambrian Bioworks directly.

The OncoSeek kit employs selective binding buffer conditions that favor short cfDNA fragments (150–200 bp) over high-molecular-weight genomic DNA. This chemistry limits gDNA carryover into the eluate, ensuring a cleaner cfDNA output essential for sensitive applications like ddPCR and NGS, where gDNA contamination can significantly reduce assay accuracy.

Yes, Cambrian Bioworks offers a tester kit upon request so labs can evaluate the OncoSeek cfDNA Extraction Kit's performance before committing to a full purchase. The full product is available in a 50-reaction pack. Pricing is available on request. You can reach the team via the contact form at cambrianbioworks.com or by calling +91 78922 39793.