Automated Stranded RNA-Seq Library Prep: Blood RNA Input Requirements

The kit reports RNA Integrity Number values from 8.6 to 9.7, validated on the Agilent Tapestation System. That level of integrity is well suited for sensitive downstream applications such as RNA-Seq, transcriptome analysis, and gene expression profiling. The workflow also includes RNase-free DNase to reduce DNA contamination and support cleaner sequencing input.

Yes. The workflow is fully automated on the Manta platform using protocol CBW-200-i4. It has a 50-minute on-deck time and uses pre-filled, ready-to-use cartridges with lysis buffers included. This setup reduces manual handling, improves reproducibility, and supports scalable processing for research and clinical laboratory environments running RNA-based assays.

Reported average RNA yield depends on blood input volume and scales linearly. At 0.5 mL input, average yield is 2.17 µg; at 1 mL, 5.64 µg; and at 2 mL, 9.61 µg. This predictable scaling helps labs plan extraction input for stranded RNA-Seq library prep, transcriptomics studies, and other workflows requiring defined RNA mass and quality.

The kit includes RNase-free DNase and 10X DNase buffer to minimize DNA contamination during RNA isolation. This is important for stranded RNA-Seq and other expression-based assays where residual genomic DNA can affect data quality. The non-reusable cartridge design also helps reduce cross-contamination risk between samples in automated workflows.

The kit is designed for whole blood and is also listed as compatible with cell cultures in the technical specifications. For this category, the primary focus is whole blood RNA extraction for sequencing and transcriptomics workflows. Labs working with blood-based gene expression, biomarker discovery, or clinical research can use the defined input range and automation format to standardize preparation.

Cartridges and buffers are stored at room temperature. Reagent R should be stored at 4°C before reconstitution and at -20°C after reconstitution. RNase-free DNase and 10X DNase buffer should be stored at -20°C before and after use. Following these storage conditions helps preserve reagent performance and maintain consistent RNA extraction quality.