ISOLATE II Genomic DNA Kit Alternatives: Automated Blood DNA Options

The standard input volume is 200 µL of whole blood, but the protocol is flexible and can be modified to accommodate volumes ranging from 200 µL up to 2 mL. An application note for a 600 µL blood starting volume protocol is also available for download from Cambrian Bioworks technical documentation.

Using 200 µL of properly collected and stored whole blood, the kit typically yields 6–15 ng/µL of genomic DNA eluted in 100 µL. Purity metrics are A260/280 of 1.8–2.0 and A260/230 of 2.0–2.2, indicating high-quality, inhibitor-free DNA suitable for sensitive downstream applications including NGS and genetic testing.

Yes. The kit is specifically optimized for NGS workflows. The high molecular weight DNA it produces, with consistent purity ratios and minimal contaminants, meets the quality requirements of leading NGS platforms. It is also compatible with PCR-based assays, microarray analysis, genetic testing, and translational research applications.

The kit supports flexible throughput of 1 to 32 samples per run on the Manta instrument. Because each pre-filled cartridge is single-use and independent, there is no batching pressure — labs can process even a single sample without wasting reagents or consumables, making it ideal for both low- and high-volume workflows.

While the kit is optimized for whole blood, the Manta instrument and cartridge system also support cell cultures and bacterial cells. Multiple sample types can be processed simultaneously in the same run, offering flexibility for diverse laboratory workflows without requiring separate instruments or protocols.

Yes. Cambrian Bioworks holds a CE-IVD mark under EU Regulation 2017/746 (IVDR) and CDSCO IVD certification for clinical DNA extraction. The company is also ISO 13485 certified for quality management in medical device manufacturing, making the kit suitable for use in regulated clinical diagnostic environments.