EBV Quantitative PCR: DNA Extraction from Blood & Plasma

A positive EBV PCR result indicates the presence of detectable Epstein-Barr Virus DNA in the sample. This can signal an active primary infection, viral reactivation in immunocompromised patients, or persistent infection. Quantitative results help distinguish low-level latent presence from clinically significant viral replication requiring medical intervention, and are used alongside clinical findings for diagnosis.

Whole blood (EDTA-collected) is the standard sample type for EBV viral load monitoring, processed using the Cambrian Whole Blood gDNA Isolation Kit. Plasma is preferred for liquid biopsy and cell-free DNA applications using the OncoSeek cfDNA Extraction Kit, which captures 150–200 bp cfDNA fragments with minimal genomic DNA contamination.

The Cambrian Whole Blood gDNA Isolation Kit completes extraction in under 30 minutes, with a lysis time of 10 minutes and a total on-deck instrument time of 28 minutes. The kit processes 200 µL to 2 mL of blood and delivers DNA with A260/280 ratios of 1.8–2.0, suitable for direct use in qPCR assays.

The OncoSeek cfDNA Extraction Kit requires as little as 1 mL of plasma to deliver 5–20× higher cfDNA yield compared to competing kits that require 2 mL. This efficiency is critical when working with limited clinical samples from pediatric patients or repeat-draw protocols in EBV and oncology monitoring workflows.

Yes. Both kits are optimized for downstream applications including qPCR, digital PCR (ddPCR), and NGS library preparation. The Whole Blood gDNA Kit delivers DNA with A260/280 of 1.8–2.0, ideal for PCR-based assays. The OncoSeek cfDNA Kit preferentially enriches 150–200 bp tumor-derived fragments compatible with sensitive mutation detection workflows.

Yes. The Cambrian Whole Blood gDNA Isolation Kit is compatible with the Manta instrument, processing 1 to 32 samples per run using pre-filled cartridges. The OncoSeek cfDNA Extraction Kit also supports automation via bead-based purification. Both systems eliminate batching pressure, making them suitable for high-throughput clinical and research diagnostic laboratories.